比較ゲノム解析研究室

De novo genome assembly of two tomato ancestors, Solanum pimpinellifolium and S. lycopersicum var. cerasiforme, by long-read sequencing.

Takei H, Shirasawa K, Kuwabara K, Toyoda A, Matsuzawa Y, Iioka S, Ariizumi T

DNA Research 2020 January 20 DOI:10.1093/dnares/dsaa029

比較ゲノム解析研究室

Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis

Amit Rai, Hideki Hirakawa, Ryo Nakabayashi, Shinji Kikuchi, Koki Hayashi, Megha Rai, Hiroshi Tsugawa, Taiki Nakaya, Tetsuya Mori, Hideki Nagasaki, Runa Fukushi, Yoko Kusuya, Hiroki Takahashi, Hiroshi Uchiyama, Atsushi Toyoda, Shoko Hikosaka, Eiji Goto, Kazuki Saito and Mami Yamazaki

Nature Communications 12, 405 (2021) DOI:10.1038/s41467-020-20508-2

The NIG international webinar by Prof. Kanemaki will be held on February 2nd at 4 pm, Eastern Time (on February 3rd at 6:00 am, Japan Standard Time). He will talk about a new genetic tool that enables rapid and reversible depletion of target proteins in living cells and animals. A Zoom link of the webinar will be obtained by free registration at the following URL (https://rois.zoom.us/meeting/register/tJAudOuprD4oHdV4dRlS6mAIdKX2F-jlAIEJ).

Time and Date:

  Eastern Standard Time (EST): 4:00 pm, February 2nd
  Pacific Standard Time (PST): 1:00 pm, February 2nd
  Greenwich Mean Time (GMT): 9:00 pm, February 2nd
  Central European Time (CET): 10:00 pm, February 2nd
  Japan Standard Time (JST): 6:00 am, February 3rd

Registration (Zoom URL will be obtained by the free registration):

  https://rois.zoom.us/meeting/register/tJAudOuprD4oHdV4dRlS6mAIdKX2F-jlAIEJ

Title:

  AID2 enables rapid target protein degradation in living mammalian cells and mice

Speaker:

  Prof. KANEMAKI, Masato
  Molecular Cell Engineering Laboratory
  National Institute of Genetics

Summary:

Genetic perturbation is a powerful way to analyze the function of proteins in living cells. For this purpose, we pioneered to develop the auxin-inducible degron (AID) technology by which a degron-fused protein can be rapidly degraded after the addition of the plant hormone auxin (Nishimura et al., Nat. Methods, 2009). By combining with CRISPR-based genome editing, it was possible to generate AID conditional mutants of human cells (Natsume et al., Cell Reports, 2016). The AID system became one of the popular genetic tools to study the function of proteins. However, leaky degradation and high doses of auxin for inducing degradation have been major drawbacks. Moreover, nobody has successfully applied the AID system to control protein degradation in living mice. We recently overcame these problems by taking advantage of chemical biology and successfully established the AID2 system (Yesbolatova et al. Nature Communications, 2020). By using AID2, we can now sharply control protein degradation in yeast, mammalian cells and mice.

  • Link to KANEMAKI laboratory
  • EurekAlert! link about the paper, Yesbolatova et al. Nature Communications, 2020
  • Link to an interview of Prof. KANEMAKI

Chairperson: MAESHIMA, Kazuhiro

　北野教授が井上学術賞を受賞することが2020年12/15に発表されました。
　http://www.inoue-zaidan.or.jp/b-01.html?eid=00045

　井上学術賞は、自然科学の基礎的研究で特に顕著な業績をあげた研究者で、50歳未満の者に与えられる賞です。北野教授は、長年行ってきた「生物多様性進化の遺伝基盤」の一連の研究業績が評価されました。2021年 2月 4日にオンラインで授賞式が行われます。

研究テーマ：生物多様性進化の遺伝基盤

　　　　　　  Genetic basis for the evolution of biodiversity

公益財団法人　井上科学振興財団

生態遺伝学研究室