Experimental Technique (by Laboratories)

-Genome Evolution • Kurokawa Group
 Technique for predicting environmental temperature based on metagenomic sequence (Metagenomic Thermometer
 Fast and accurate taxonomic assignment tool of metagenomic dataVITCOMIC2
 Latent environment allocation of microbial community dataLEA
 Ultra-fast amino acid sequence similarity search server against public metagenomesPZLAST

-Genome Diversity • Mori Group
 Technique for predicting environmental temperature based on metagenomic sequencesMetagenomic Thermometer
 Ultra-fast amino acid sequence similarity search server against public metagenomesPZLAST

-Genome Informatics • Nakamura Group
 Practical Assembly Guideline for Genomes with Heterozygosity
 ‘one-click’ identification of causative on webMudi
 Prokaryotic genome annotation pipeline for data submission to DDBJDFAST

-Brain Function • Hirata Group
 Neuronal birthdate tagging method
 “NeuroGT Database”, a brain atlas of neurogenic tagging CreER driversNeuroGT

-Molecular and Developmental Biology • Kawakami Group
 Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd
 Seeing the visual world in the brain in real time
 Simple and efficient multiple sgRNA expression system in zebrafish
 Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
 Virtual reality system to analyze neural activity and behavior in adult zebrafish

-Mouse Genomics Resource • Koide Group
 Genome editing in wild mouse strains
 Development of a freeware that enables automatic characterization of social behavior in miceDuoMouse

-Symbiosis and Cell Evolution • Miyagishima Group
 Protein Knockdown Method in Photosynthetic Organisms


-Microbial Physiology • Niki Group
 High efficient cloning technology using iVEC method

-Plant Cytogenetics • Nonomura Group
 Visualization of autophagy
 3D analysis of complex structures in living things
  (Research paper)(You can see the 3D images of several organisms.)

-Mammalian Development • Saga Group *Professor Saga retired in April 2022.
 Simple non-arbitrary method to quantify fluorescent images
 Genetics without making mouse lines

-Model Fish Genetics • Sakai Group
 Establishment of a zebrafish inbred strain

 In vitro storage of functional sperm at room temperature in zebrafish and medaka
 Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.

-Multicellular Organization Laboratory • Sawa Group
 Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system

-Mammalian Genetics • Shiroishi Group *Now operated at RIKEN BRC
 User-friendly NIG mouse genome databaseNIG_MoG
 Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the publicNIG_MoG2

-DNA Data Analysis • Ikeo Group
 One-stop platform for NGS data analysisMaser

-Human Genetics • Inoue Group
 Comprehensive discovery of CRISPR-targeted terminally redundant sequences

-Molecular Cell Engineering • Kanemaki Group
 AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
 Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
 Simple method for generation of yeast conditional mutants by use of the AID technology
 New toolkit for tagging and a degradation inhibitor for the AID system

-Genome Dynamics • Maeshima Group
 Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
 Method for telomere-specific chromatin capture

Database Center for Life Science (DBCLS)
 CRISPRdirect — Rational design of CRISPR/Cas target
 Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*
*These tools are developed jointly with the National Bioscience Database Center (NBDC) of JST.

NIG Collaboration Grants


  • Twitter
  • facebook
  • youtube