Experimental Technique (by Laboratories)
-Genome Evolution • Kurokawa Group
Technique for predicting environmental temperature based on metagenomic sequence （Metagenomic Thermometer
）
Fast and accurate taxonomic assignment tool of metagenomic data（VITCOMIC2）
Latent environment allocation of microbial community data（LEA）
Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
-Genome Diversity • Mori Group
Technique for predicting environmental temperature based on metagenomic sequences（Metagenomic Thermometer）
Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
-Genome Informatics • Nakamura Group
Practical Assembly Guideline for Genomes with Heterozygosity
‘one-click’ identification of causative on web（Mudi）
Prokaryotic genome annotation pipeline for data submission to DDBJ（DFAST）
-Brain Function • Hirata Group
Neuronal birthdate tagging method
“NeuroGT Database”, a brain atlas of neurogenic tagging CreER drivers（NeuroGT）
-Molecular and Developmental Biology • Kawakami Group
Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd
Seeing the visual world in the brain in real time
Simple and efficient multiple sgRNA expression system in zebrafish
Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
Virtual reality system to analyze neural activity and behavior in adult zebrafish
-Mouse Genomics Resource • Koide Group
Genome editing in wild mouse strains
Development of a freeware that enables automatic characterization of social behavior in mice
（DuoMouse）
-Microbial Physiology • Niki Group
High efficient cloning technology using iVEC method
-Plant Cytogenetics • Nonomura Group
Visualization of autophagy
3D analysis of complex structures in living things
(Research paper)(You can see the 3D images of several organisms.)
-Mammalian Development • Saga Group *Professor Saga retired in April 2022.
Simple non-arbitrary method to quantify fluorescent images
Genetics without making mouse lines
-Model Fish Genetics • Sakai Group
In vitro storage of functional sperm at room temperature in zebrafish and medaka
Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.
-Multicellular Organization Laboratory • Sawa Group
Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
-Mammalian Genetics • Shiroishi Group *Now operated at RIKEN BRC
User-friendly NIG mouse genome database（NIG_MoG）
Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the public（NIG_MoG2）
-DNA Data Analysis • Ikeo Group
One-stop platform for NGS data analysis（Maser）
-Human Genetics • Inoue Group
Comprehensive discovery of CRISPR-targeted terminally redundant sequences
-Molecular Cell Engineering • Kanemaki Group
AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
Simple method for generation of yeast conditional mutants by use of the AID technology
New toolkit for tagging and a degradation inhibitor for the AID system
-Genome Dynamics • Maeshima Group
Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
Method for telomere-specific chromatin capture
Database Center for Life Science （DBCLS)
CRISPRdirect — Rational design of CRISPR/Cas target*
Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*
*These tools are developed jointly with the National Bioscience Database Center (NBDC) of JST.
