Experimental Technique (by Categories)

 Seeing the visual world in the brain in real time
 Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
 3D analysis of complex structures in living things
 (Research paper)(You can see the 3D images of several organisms.)
 Visualization of autophagy
 Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
 Virtual reality system to analyze neural activity and behavior in adult zebrafish

 Practical Assembly Guideline for Genomes with Heterozygosity
 Method for telomere-specific chromatin capture
 Comprehensive discovery of CRISPR-targeted terminally redundant sequences

-Modification of Genes
 AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
 Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
 Simple method for generation of yeast conditional mutants by use of the AID technology
 Genome editing in wild mouse strains
 High efficient cloning technology using iVEC method
 Genetics without making mouse lines
 New toolkit for tagging and a degradation inhibitor for the AID system
 Simple and efficient multiple sgRNA expression system in zebrafish
 Neuronal birthdate tagging method

-DNA recombination
 Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd

-Cell Culture

 In vitro storage of functional sperm at room temperature in zebrafish and medaka
 Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.

-Analytical Tool
 Technique for predicting environmental temperature based on metagenomic sequencesMetagenomic Thermometer
 Fast and accurate taxonomic assignment tool of metagenomic dataVITCOMIC2
 Latent environment allocation of microbial community dataLEA
 Ultra-fast amino acid sequence similarity search server against public metagenomesPZLAST
 ‘one-click’ identification of causative on webMudi
 Development of a freeware that enables automatic characterization of social behavior in miceDuoMouse
 Simple non-arbitrary method to quantify fluorescent images
 One-stop platform for NGS data analysisMaser
 Prokaryotic genome annotation pipeline for data submission to DDBJDFAST
 CRISPRdirect — Rational design of CRISPR/Cas target

 Metabolome databases for analyzing sample-specific unknown metabolitesFoodMR )(PlantMR )(ThingMR
 User-friendly NIG mouse genome databaseNIG_MoG
 Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the publicNIG_MoG2
 “NeuroGT Database”, a brain atlas of neurogenic tagging CreER driversNeuroGT
 Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*
 *Database Center for Life Science (DBCLS)

NIG Collaboration Grants

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