Experimental Technique (by Categories)

Seeing the visual world in the brain in real time
Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
3D analysis of complex structures in living things (Research paper)(You can see the 3D images of several organisms.)
Visualization of autophagy
Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
Virtual reality system to analyze neural activity and behavior in adult zebrafish

Practical Assembly Guideline for Genomes with Heterozygosity
Method for telomere-specific chromatin capture
Comprehensive discovery of CRISPR-targeted terminally redundant sequences

-Modification of Genes
AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
Simple method for generation of yeast conditional mutants by use of the AID technology
Genome editing in wild mouse strains
High efficient cloning technology using iVEC method
Genetics without making mouse lines
New toolkit for tagging and a degradation inhibitor for the AID system
Simple and efficient multiple sgRNA expression system in zebrafish
Neuronal birthdate tagging method

-DNA recombination
Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd

-Cell Culture
Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.

-Analytical Tool
Technique for predicting environmental temperature based on metagenomic sequencesMetagenomic Thermometer
Fast and accurate taxonomic assignment tool of metagenomic dataVITCOMIC2
Latent environment allocation of microbial community dataLEA
Ultra-fast amino acid sequence similarity search server against public metagenomesPZLAST
‘one-click’ identification of causative on webMudi
Development of a freeware that enables automatic characterization of social behavior in miceDuoMouse
Simple non-arbitrary method to quantify fluorescent images
One-stop platform for NGS data analysisMaser
Prokaryotic genome annotation pipeline for data submission to DDBJDFAST
CRISPRdirect — Rational design of CRISPR/Cas target*

Metabolome databases for analyzing sample-specific unknown metabolitesFoodMR)(PlantMR)(ThingMR
Systematic identification of regulatory elements derived from human endogenous retrovirusesdbHERV-REs
User-friendly NIG mouse genome databaseNIG_MoG
Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the publicNIG_MoG2
“NeuroGT Database”, a brain atlas of neurogenic tagging CreER driversNeuroGT
Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*

*Database Center for Life Science (DBCLS)

NIG Collaboration Grants

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