Observation/Analysis/Modification of Gene/Development of bioresources/DNA recombination/
Cell Culture/Analytical Tool/Database

-Observation
　Seeing the visual world in the brain in real time
　Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
　3D analysis of complex structures in living things
　(Research paper)(You can see the 3D images of several organisms.)
　Visualization of autophagy
　Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
　Virtual reality system to analyze neural activity and behavior in adult zebrafish

-Analysis
　Practical Assembly Guideline for Genomes with Heterozygosity
　Method for telomere-specific chromatin capture
　Comprehensive discovery of CRISPR-targeted terminally redundant sequences

-Modification of Genes
　Protein Knockdown Method in Photosynthetic Organisms
　AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
　Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
　Simple method for generation of yeast conditional mutants by use of the AID technology
　Genome editing in wild mouse strains
　High efficient cloning technology using iVEC method
　Genetics without making mouse lines
　New toolkit for tagging and a degradation inhibitor for the AID system
　Simple and efficient multiple sgRNA expression system in zebrafish
　Neuronal birthdate tagging method

-Development of bioresources
　Establishment of a zebrafish inbred strain

-DNA recombination
　Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd

-Cell Culture

　In vitro storage of functional sperm at room temperature in zebrafish and medaka
　Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.

-Analytical Tool
　Technique for predicting environmental temperature based on metagenomic sequences（Metagenomic Thermometer）
　Fast and accurate taxonomic assignment tool of metagenomic data（VITCOMIC2）
　Latent environment allocation of microbial community data（LEA）
　Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
　‘one-click’ identification of causative on web（Mudi）
　Development of a freeware that enables automatic characterization of social behavior in mice（DuoMouse）
　Simple non-arbitrary method to quantify fluorescent images
　One-stop platform for NGS data analysis（Maser）
　Prokaryotic genome annotation pipeline for data submission to DDBJ（DFAST）
　CRISPRdirect — Rational design of CRISPR/Cas target* 

-Database
　Metabolome databases for analyzing sample-specific unknown metabolites（FoodMR ）（PlantMR ）（ThingMR ）
　User-friendly NIG mouse genome database（NIG_MoG）
　Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the public（NIG_MoG2）
　“NeuroGT Database”, a brain atlas of neurogenic tagging CreER drivers（NeuroGT）
　Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*
　
*Database Center for Life Science （DBCLS)

NIG Collaboration Grants