A virtual reality system to analyze neural activity and behavior in adult zebrafish

Press Release

A virtual reality system to analyze neural activity and behavior in adult zebrafish

Kuo-Hua Huang, Peter Rupprecht, Thomas Frank, Koichi Kawakami, Tewis Bouwmeester and Rainer W. Friedrich

Nature methods 02 March 2020 DOI:10.1038/s41592-020-0759-2

Press release (In Japanese only)

Virtual realities are powerful tools to analyze and manipulate interactions between animals and their environment and to enable measurements of neuronal activity during behavior. In many species, however, optical access to the brain and/or the behavioral repertoire are limited. We developed a high-resolution virtual reality for head-restrained adult zebrafish, which exhibit cognitive behaviors not shown by larvae. We noninvasively measured activity throughout the dorsal telencephalon by multiphoton calcium imaging. Fish in the virtual reality showed regular swimming patterns and were attracted to animations of conspecifics. Manipulations of visuo-motor feedback revealed neurons that responded selectively to the mismatch between the expected and the actual visual consequences of motor output. Such error signals were prominent in multiple telencephalic areas, consistent with models of predictive processing. A virtual reality system for adult zebrafish therefore provides opportunities to analyze neuronal processing mechanisms underlying higher brain functions including decision making, associative learning, and social interactions.

Source: Kuo-Hua Huang , et al., Published: 02 March 2020
DOI: 10.1038/s41592-020-0759-2

Figure1

Fig: Virtual reality system to analyze neural activity and behavior in adult zebrafish
A: Head fixation with L-shaped bars
B: Virtual reality projected by three projectors onto a panoramic screen
C: Setup for virtual reality and two-photon imaging
D: Virtual reality seen by the head-fixed zebrafish


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