Genome Informatics Laboratory • Nakamura Group Division of Cytogenetics • Kobayashi Group

Genetics is a powerful approach to discovery of the molecular mechanisms underlying biological phenomena. However, experiments for identification of mutations by classical genetics is a time-consuming and laborious process. Modern whole-genome sequencing, coupled with bioinformatics analysis, has enabled fast and cost-effective mutation identification. However, for many experimental researchers, bioinformatics analysis is still a difficult aspect of whole-genome sequencing. To address this issue, we developed a browser-accessible and easy-to-use bioinformatics tool called Mutation discovery (Mudi; http://naoii.nig.ac.jp/mudi_top.html). Users can run Mudi analysis with ‘one-click’ operation in a web browser after uploading genome resequencing data. Mudi simplifies analysis of whole-genome sequencing and thereby expands the possibilities for systematic forward-genetic approaches in various organisms.

Workflow of “Mudi” system. Genomic DNA is prepared from pooled mutants by pooled-linkage analysis. Mudi calls prioritized mutation candidates by bioinformatics analysis using genome-resequencing data.