SCHENGEN receptor module drives localized ROS production and lignification in plant roots
Satoshi Fujita, Damien De Bellis, Kai H Edel, Philipp Köster, Tonni Grube Andersen, Emanuel Schmid-Siegert, Valérie Denervaud Tendon, Alexander Pfister, Peter Marhavý, Robertas Ursache, Verónica G. Doblas, Marie Barberon, Jean Daraspe, Audrey Creff, Gwyneth Ingram, Jörg Kudla, Niko Geldner
EMBO J (2020)e103894 DOI:10.15252/embj.2019103894
Reactive oxygen species (ROS) impact many physiological processes in animals or plants, but its production by NADPH oxidases is strictly regulated because of extremely high reactivity. Many plant receptor pathways are known as critical regulators of ROS production, but how they spatially control ROS production is yet to be clarified.
Fujita et al. (2020) established a phospho-signaling pathway that integrates direct, rapid activation of ROS production with positional information and transcriptional changes to form proper diffusion barriers. Firstly, the authors presented a direct connection from a peptide-receptor complex to NADPH oxidases via a membrane-anchored kinase by biochemically. Next, the authors focused on the membrane-anchored kinase that localizes on the plasma membrane in a polar fashion. Manipulation of the kinase localization successfully showed that this biased distribution of the kinase protein gave the positional cue, which enables the SCHENGEN pathway to activate only one side of the Casparian strip at micrometer-scale precision. This work would contribute to highlight how other receptor pathways could control local ROS production.
This work was mainly done by Dr. Satoshi Fujita (former postdoctoral fellow in University of Lausanne, currently NIG research fellow) in Prof. Niko Geldner’s group with a collaboration with the Central imaging, Electron microscope, and Genomics facilities of University of Lausanne, Swiss Institute of bioinformatics, Prof. Jörg Kudla’s group at University of Munster and Dr. Gwyneth Ingram’s group at University of Lyon.
This work was aided by grants no. 31003A_156261 and 310030E_176090 (N.G.), from the Swiss National Science Foundation, an ERC Consolidator Grant (616228-ENDOFUN) (N.G.), DFG grant (Ku931/14-1 (J.K.)) FEBS long-term fellowship (P.M.), EMBO long-term fellowship (R.U., M.B.), Marie Curie postdoctoral fellowship (T.G.A,), Fundacion Alfonso Martin Escudero fellowship (V.G.D.), and a Japan Society for the Promotion of Science (JSPS) fellowship (S.F.).
Figure: Plant roots have a lignin-based diffusion barrier, namely Casparian strips. Localized ROS production (EM pictures) by asymmetrical signal activation (schematic model) sustains functional barrier formation.