A specifically patterned cell wall is a determinant of plant cell shape. However, the precise mechanism underlying the cell wall patterning is still elusive. The main purpose of our study is to reveal how plant cells establish proper cell wall patterns. We focus on xylem vessel cells that deposit secondary cell walls in various patterns such as spiral, reticulate, and pitted patterns. By using our xylogenic cell culture system and pattern reconstruction assay, we have revealed that cortical cytoskeletons and Rho GTPases play a central role in the secondary cell wall patterning.
(A) Xylem vessels develop various secondary cell wall patterns. (B) Xylogenic Arabidopsis cultured cells. Red signals indicate secondary cell walls. (C) Cortical microtubules in the differentiating xylem cell. (D) Secondary cell walls (yellow) and plasma membrane domains (green). (E) Reconstruction assay for secondary cell wall patterns. Magenta: plasma membrane domains. Green: microtubules.
Oda, Y., and Fukuda, H. (2013). Rho of plant GTPase signaling regulates the behavior of Arabidopsis kinesin-13A to establish secondary cell wall patterns. Plant Cell 25, 4439-4450.
Oda, Y., and Fukuda, H. (2012). Initiation of cell wall pattern by a Rho- and microtubule-driven symmetry breaking. Science 337, 1333-1336.
Oda, Y., Iida, Y., Kondo, Y., and Fukuda, H. (2010). Wood cell-wall structure requires local 2D-microtubule disassembly by a novel plasma membraneanchored protein. Current Biology 20, 1197-1202.