A specifically patterned cell wall is a determinant of plant cell shape. However, the precise mechanism underlying the cell wall patterning is still elusive. The main purpose of our study is to reveal how plant cells establish proper cell wall patterns. We focus on xylem vessel cells that deposit secondary cell walls in various patterns such as spiral, reticulate, and pitted patterns. By using our xylogenic cell culture system and pattern reconstruction assay, we have revealed that cortical cytoskeletons and Rho GTPases play a central role in the secondary cell wall patterning.
(A) Xylem vessels develop various secondary cell wall patterns. (B) Xylogenic Arabidopsis cultured cells. Red signals indicate secondary cell walls. (C) Cortical microtubules in the differentiating xylem cell. (D) Secondary cell walls (yellow) and plasma membrane domains (green). (E) Reconstruction assay for secondary cell wall patterns. Magenta: plasma membrane domains. Green: microtubules.
Sasaki, T., Fukuda, H., and Oda, Y. (2017). CORTICAL MICROTUBULE DISORDERING1 is required for secondary cell wall patterning in xylem vessels. Plant Cell 29, 3123-3139.
Sugiyama, Y., Wakazaki, M., Toyooka, K., Fukuda, H., and Oda, Y. (2017). A novel plasma membrane-anchored protein regulates xylem cell-wall deposition through microtubule-dependent lateral inhibition of Rho GTPase domains. Curr Biol 27, 2522-2528.
Oda, Y., Iida, Y., Nagashima, Y., Sugiyama, Y., and Fukuda, H. (2015). Novel coiled-coil proteins regulate exocyst association with cortical microtubules in xylem cells via the Conserved Oligomeric Golgi-complex 2 protein. Plant Cell Physiol 56, 277-286.