A specifically patterned cell wall is a determinant of plant cell shape. However, the precise mechanism underlying the cell wall patterning is still elusive. The main purpose of our study is to reveal how plant cells establish proper cell wall patterns. We focus on xylem vessel cells that deposit secondary cell walls in various patterns such as spiral, reticulate, and pitted patterns. By using our xylogenic cell culture system and pattern reconstruction assay, we have revealed that cortical cytoskeletons and Rho GTPases play a central role in the secondary cell wall patterning.
(A) Xylem vessels develop various secondary cell wall patterns. (B) Xylogenic Arabidopsis cultured cells. Red signals indicate secondary cell walls. (C) Cortical microtubules in the differentiating xylem cell. (D) Secondary cell walls (yellow) and plasma membrane domains (green). (E) Reconstruction assay for secondary cell wall patterns. Magenta: plasma membrane domains. Green: microtubules.
Sasaki T, Tsutsumi M, Otomo K, Murata T, Yagi N, Nakamura M, Nemoto T, Hasebe M, Oda Y. (2019). A Novel Katanin-Tethering Machinery Accelerates Cytokinesis. Current Biology doi: 10.1016/j.cub.2019.09.049.
Sugiyama Y, Nagashima Y, Wakazaki M, Sato M, Toyooka K, Fukuda H, Oda Y. (2019). A Rho-actin signaling pathway shapes cell wall boundaries in Arabidopsis xylem vessels. Nature Communications. 10:468. doi: 10.1038/s41467-019-08396-7.
Nagashima Y, Tsugawa S, Mochizuki A, Sasaki T, Fukuda H, Oda Y. (2018). A Rho-based reaction-diffusion system governs cell wall patterning in metaxylem vessels. Scientific Reports. 8:11542. doi: 10.1038/s41598-018-29543-y.