2013/07/31

Mobilization of a plant transposon by expression of the transposon-encoded anti-silencing factor

Division of Agricultural Genetics • Kakutani Group

Mobilization of a plant transposon by expression of the transposon-encoded anti-silencing factor
Yu Fu, Akira Kawabe, Mathilde Etcheverry, Tasuku Ito, Atsushi Toyoda, Asao Fujiyama, Vincent Colot, Yoshiaki Tarutani, Tetsuji Kakutani
EMBO Journal, advance online publication 30 July 2013; doi:10.1038/emboj.2013.169

Transposable elements (TEs) have a major impact on genome evolution, but they are potentially deleterious, and most of them are silenced by epigenetic mechanisms such as DNA methylation. Here we report the characterization of a TE encoding an activity to counteract epigenetic silencing by the host. In Arabidopsis thaliana, we identified a mobile copy of the Mutator-like element (MULE) with degenerated terminal inverted repeats (TIRs). This TE, named Hiun (Hi), is silent in wild type plants, but it transposes when DNA methylation is abolished. When a Hi transgene was introduced into the wild type background, it induced excision of the endogenous Hi copy, suggesting that Hi is the autonomously mobile copy. In addition, the transgene induced loss of DNA methylation and transcriptional activation of the endogenous Hi. Most importantly, the trans-activation of Hi depends on a Hi-encoded protein different from the conserved transposase. Proteins related to this anti-silencing factor, which we named VANC, are widespread in the non-TIR MULEs and may have contributed to the recent success of these TEs in natural Arabidopsis populations.

(A) Structure of the plant transposon Hiun (Hi). This transposon encodes for the transposase and the anti-silencing factor. The antisilencing factor induces demethylation and transcriptional de-repression.
(B) DNA methylation of endogenous Hi compared between non-transgenic plant (blue) and transgenic plant expressing Hi transgene (red). The transgene induced demethylaton of endogenous copy, especially at non-CpG sites.


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