Sawa Group • Multicellular Organization Laboratory

Generation of cellular diversity by asymmetric cell division

Faculty




Research Summary

Various cells including stem cells undergo asymmetric cell divisions to produce daughter cells with distinct cell fates. Most cells in C. elegans have the same anterior-posterior polarity in terms of localizations of Wnt signaling components such as β-catenin, and divide asymmetrically to produce a variety of cell types. Similar asymmetric localization was reported in mouse ES cells. We are studying how each cell knows the correct orientation, how it divides asymmetrically and how the daughter cells acquire specific cell fates.

Asymmetric localization of β-catenin before (A) and at telophase (B) of asymmetric division. Arrowheads indicate cell boundary. (C) Polarity orientation (arrows) of epithelial stem cells (light blue) is redundantly controlled by three Wnt proteins (CWN-1, CWN-2, EGL-20).

Publications

Sugioka, K., Fielmich, L. E., Mizumoto, K., Bowerman, B., van den Heuvel, S., Kimura, A., and Sawa, H. (2018). Tumor suppressor APC is an attenuator of spindle-pulling forces during C.elegans asymmetric cell division. Proc Natl Acad Sci USA, doi: 10.1073/pnas.1712052115.

Sugioka, K., Mizumoto, K., and Sawa, H. (2011). Wnt regulates spindle asymmetry to generate asymmetric nuclear β-catenin C. elegans. Cell 146, 942-954.

Yamamoto, Y., Takeshita, H., and Sawa, H. (2011). Multiple Wnts redundantly control polarity orientation in Caenorhabditis elegans epithelial stem cells. PLoS Genet 7, e1002308.


  • Twitter
  • facebook
  • youtube