Niki Group • Microbial Physiology Laboratory

Genetic dissection of the cell division mechanism using single-cellular model organisms

Faculty



Research Summary

Bacteria and yeast are important model organisms to elucidate the fundamental mechanisms of cell proliferation. Our laboratory studies the mechanisms behind the cell division cycle and adaptations to external stresses under environments. We focused on compaction of chromosomal DNA as a nucleoid inside a tiny bacterial cell during cell division. Bacterial condensin is an essential factor for packaging of a nucleoid to properly segregate into daughter cells. Also, we study on hyphal development and growth by using a new model organism, Schizosaccharomyces japonicus. We established new investigative methodologies to investigated S. japonicus.

https://shigen.nig.ac.jp/ecoli/strain/
https://shigen.nig.ac.jp/bsub/

Model of the mechanism of iVEC (in vivo E. coli cloning). Work flow of “one-tube” transformation. Preparation of competent cells and introduction of DNA can be done with a single tube. For more information: https://shigen.nig.ac.jp/bsub/

Publications

Nozaki S, Niki H. Exonuclease III (XthA) Enforces In Vivo DNA Cloning of Escherichia coli To Create Cohesive Ends. J Bacteriol. 2019 Feb 11;201(5). pii: e00660-18.

Nozaki S, Niki H. Exonuclease III (XthA) Enforces In Vivo DNA Cloning of Escherichia coli To Create Cohesive Ends. J Bacteriol. 2019 Feb 11;201(5). pii: e00660-18.

Nozaki S, Furuya K, Niki H. The Ras1-Cdc42 pathway is involved in hyphal development of Schizosaccharomyces japonicus. FEMS Yeast Res. 2018 Jun 1;18(4).

Yano K, Niki H. Multiple cis-Acting rDNAs Contribute to Nucleoid Separation and Recruit the Bacterial Condensin Smc-ScpAB. Cell Rep. 2017 Oct 31;21(5):1347-1360.


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