Microbial Genetics Laboratory • Niki Group

Genetic dissection of the cell division mechanism using single-cellular model organisms


Research Summary

Bacteria and yeasts are suitable model organisms to understand the fundamental mechanisms on cell proliferation. Our laboratory studies the mechanisms behind chromosome and plasmid DNA dynamics in the cell or the mechanism underlies cell shape formation. Genetic methods as well as cell-biological methods were used to observe those intracellular events. We have made several novel observations in cell proliferation mechanism by using fluorescentbased protein or DNA imaging. Especially S. japonicus yeast suits for those cell biological analyses, and studies of hyphal growth and cell cycle add special value on this organism. Novel genetic system has been elucidated by recent our works.


Hyphal cells of Schizosaccharomyces japonicas. Green fluorescent protein indicates nuclei and red fluorescent protein indicates cytosolic region.


Niki, H., and Yano, K. (2016). In vitro topological loading of bacterial condensing MukB on DNA, preferentially single-stranded DNA rather than double-stranded DNA. Sci Rep 6, 29469.

Aoki, K., Furuya, K., and Niki, H. (2016). Schizosaccharomyces japonicus: a distinct dimorphic yeast among the fission yeast. CSHL Protocol doi:10.1101/ pdb.top082651.

Monteiro, D.C., Patel, V., Bartlett, C.P., Nozaki, S., Grant, T.D., Gowdy, J.A., Thompson, G.S., Kalverda, A.P., Snell, E.H., Niki, H., Pearson, A.R., and Webb, M.E. (2015). The structure of the PanD/PanZ protein complex reveals negativefeedback regulation of pantothenate biosynthesis by coenzyme A. Chem Biol 22, 492-503.

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