The genetic basis for individual differences in complex traits is still unclear. In order to clarify the mechanisms related to behavioral diversity, we are using a series of wild-derived mouse strains. Wild derived strains exhibit a prominent degree of wildness and phenotypic diversity among them. We are also developing efficient genome editing methodologies in rodents with CRISPR/Cas9. We are identifying genes related to behavioral diversity using these tools, and are aiming to understand the role of these genes in the molecular, cellular, and neural mechanisms that underlie this behavioral diversity.
We applied selective breeding on wild stock of mice and established genetically tamed mice. As a result of genetic analyses, we found a genomic signature of selection on chromosome 11. The region is syntenic to the genomic region which are selected during dog domestication. These results will be useful for domestication of other animals.
Matsumoto, Y., Goto, T., Nishino, J., Nakaoka, H., Tanave, A., Takano-Shimizu, T., Mott, R. F., and Koide, T. (2017). Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness. Sci Rep 7, 4607.
Horii, Y., Nagasawa, T., Sakakibara, H., Takahashi, A., Tanave, A., Matsumoto, Y., Nagayama, H., Yoshimi, K., Yasuda, M. T., Shimoi, K., and Koide, T. (2017). Hierarchy in the home cage affects behaviour and gene expression in grouphoused C57BL/6 male mice. Sci Rep 7, 6991.
Takahashi, A., Lee, R.X., Iwasato, T., Itohara, S., Arima, H., Bettler, B., Miczek, K.A., and Koide, T. (2015). Glutamate input in the dorsal raphe nucleus as a determinant of escalated aggression in male mice. J Neurosci 35, 6452-6463.