The genetic basis for individual differences in complex traits is still unclear. In order to clarify the mechanisms related to behavioral diversity, we are using a series of wild-derived mouse strains. Wild derived strains exhibit a prominent degree of wildness and phenotypic diversity among them. We are also developing efficient genome editing methodologies in rodents with CRISPR/Cas9. We are identifying genes related to behavioral diversity using these tools, and are aiming to understand the role of these genes in the molecular, cellular, and neural mechanisms that underlie this behavioral diversity.
We applied selective breeding on wild stock of mice and established genetically tamed mice. As a result of genetic analyses, we found a genomic signature of selection on chromosome 11. The region is syntenic to the genomic region which are selected during dog domestication. Currently, we are trying to apply this method to domestication of large rodents used for food in Africa.
Tanave A, Imai Y, Koide T. Nested retrotransposition in the East Asian mouse genome causes the classical nonagouti mutation. Commun Biol. 2019 Aug 2;2(1):283.
Matsumoto Y, Goto T, Nishino J, Nakaoka H, Tanave A, Takano-Shimizu T, Mott RF, Koide T. Selective breeding and selection mapping using a novel wild-derived heterogeneous stock of mice revealed two closely-linked loci for tameness. SciRep. 2017 Jul 4;7(1):4607.
Horii Y, Nagasawa T, Sakakibara H, Takahashi A, Tanave A, Matsumoto Y, Nagayama H, Yoshimi K, Yasuda MT, Shimoi K, Koide T. Hierarchy in the home cage affects behaviour and gene expression in group-housed C57BL/6 male mice. Sci Rep. 2017 Aug 1;7(1):6991.
Hirata H, Takahashi A, Shimoda Y, Koide T. Caspr3-Deficient Mice Exhibit Low Motor Learning during the Early Phase of the Accelerated Rotarod Task. PLoS One. 2016 Jan 25;11(1):e0147887.