The genetic basis for individual differences in complex traits is still unclear. In order to clarify the mechanisms related to behavioral diversity, we are using a series of wild-derived mouse strains. Wild derived strains exhibit a prominent degree of wildness and phenotypic diversity among them. We are also developing efficient genome editing methodologies in rodents with CRISPR/Cas9. We are identifying genes related to behavioral diversity using these tools, and are aiming to understand the role of these genes in the molecular, cellular, and neural mechanisms that underlie this behavioral diversity.
(A) Notable strain differences of behavioral patterns between wild-derived and laboratory strains using a variety of behavioral studies.
(B) Efficient genome editing technologies in rodents with CRISPR/Cas9 for behavioral research.
Yoshimi, K., Kunihiro, Y., Kaneko, T., Nagahora, H., Voigt, B., and Mashimo, T. (2016). ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Nat Commun 7, 10431.
Hirata, H., Takahashi, A., Shimoda, Y., and Koide, T. (2016). Caspr3-deficient mice exhibit low motor learning during the early phase of the accelerated rotarod task. PLoS One 11, e0147887.
Takahashi, A., Lee, R.X., Iwasato, T., Itohara, S., Arima, H., Bettler, B., Miczek, K.A., and Koide, T. (2015). Glutamate input in the dorsal raphe nucleus as a determinant of escalated aggression in male mice. J Neurosci 35, 6452-6463.