Our research interest is to know how a long string of genomic DNA is three-dimensionally organized in the cell, and how the genome is read out for cellular proliferation, differentiation and development. For this purpose, we are using a unique combination of molecular cell biology and biophysics, such as single molecule imaging, superresolution imaging, X-ray scattering and computational simulation.
Chromatin consists of irregularly folded 10-nm fibers and forms numerous chromatin domains in the cell nuclei. Chromatin dynamically behaves like “liquid”. NPC, nuclear pore complex; NE, nuclear envelope.
Maeshima, K., Ide, S., Hibino, K., and Sasai, M. (2016). Liquid-like behavior of chromatin. Curr Opin Genet 37, 36-45.
Maeshima, K., Rogge, R., Tamura, S., Joti, Y., Hikima, T., Szerlong, H., Krause, C., Herman, J., Seidel, E., DeLuca, J., Ishikawa, T., and Hansen, J.C. (2016). Nucleosomal arrays self-assemble into supramolecular globular structures lacking 30-nm fibers. EMBO J 35, 1115-1132.
Sasaki, A., Ide, S., Kawamoto, Y., Bando, T., Murata, Y., Shimura, M., Yamada, K., Hirata, A., Nokihara, K., Hirata, T., Sugiyama, H., and Maeshima, K. (2016). Telomere Visualization in Tissue Sections using Pyrrole-Imidazole Polyamide Probes. Sci Rep 6, 29261.