Our research interest is to know how a long string of human genome is three-dimensionally organized in the cell, and how the human genome is read out for cellular proliferation, differentiation and development. For this purpose, we are using a unique combination of molecular cell biology and biophysics, such as single molecule imaging, superresolution imaging, X-ray scattering and computational simulation.
Human genome chromatin consists of irregularly folded 10-nm fibers and forms numerous chromatin domains in the cell nuclei. Chromatin dynamically behaves like “liquid”. NPC, nuclear pore complex; NE, nuclear envelope.
Maeshima K, Hibino K, Hudson DF. Condensins under the microscope. J Cell Biol. 2018 Jul 2;217(7):2229-2231.
Maeshima K, Matsuda T, Shindo Y, Imamura H, Tamura S, Imai R, Kawakami S, Nagashima R, Soga T, Noji H, Oka K, Nagai T. A Transient Rise in Free Mg(2+) Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6.
Imai R, Nozaki T, Tani T, Kaizu K, Hibino K, Ide S, Tamura S, Takahashi K, Shribak M, Maeshima K. Density imaging of heterochromatin in live cells using orientationindependent- DIC microscopy. Mol Biol Cell. 2017 Nov 7;28(23):3349-3359.
Nozaki T, Imai R, Tanbo M, Nagashima R, Tamura S, Tani T, Joti Y, Tomita M, Hibino K, Kanemaki MT, Wendt KS, Okada Y, Nagai T, Maeshima K. Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging. Mol Cell. 2017 Jul 20;67(2):282-293.e7.