Our research interest is to know how a long string of genomic DNA is three-dimensionally organized in the cell, and how the genome is read out for cellular proliferation, differentiation and development. For this purpose, we are using a unique combination of molecular cell biology and biophysics, such as single molecule imaging, superresolution imaging, X-ray scattering and computational simulation.
Chromatin consists of irregularly folded 10-nm fibers and forms numerous chromatin domains in the cell nuclei. Chromatin dynamically behaves like “liquid”. NPC, nuclear pore complex; NE, nuclear envelope.
Maeshima, K., Matsuda, T., Shindo, Y., Imamura, H., Tamura, S., Imai, R., Kawakami, S., Nagashima, R., Soga, T., Noji, H., Oka, K., and Nagai, T. (2018). A transient rise in free Mg(2+) ions released from ATP-Mg hydrolysis contributes to mitotic chromosome condensation. Curr Biol 28, 444-451.
Imai, R., Nozaki, T., Tani, T., Kaizu, K., Hibino, K., Ide, S., Tamura, S., Takahashi, K., Shribak, M., and Maeshima, K. (2017). Density imaging of heterochromatin in live cells using orientation-independent-DIC microscopy. Mol Biol Cell 28, 3349-3359.
Nozaki, T., Imai, R., Tanbo, M., Nagashima, R., Tamura, S., Tani, T., Joti, Y., Tomita, M., Hibino, K., Kanemaki, M. T., Wendt, K. S., Okada, Y., Nagai, T., and Maeshima, K. (2017). Dynamic organization of chromatin domains revealed by super-resolution live-cell imaging. Mol Cell 67, 282-293.