Caenorhabditis elegans is the first multicellular organism whose entire genome sequence has been determined, and its transcriptome has been extensively investigated. In addition, easy ways are available to determine gene expression patterns and to generate gene-modified strains, which are necessary for reverse genetic analysis. Based on these features, we aim at elucidating gene regulatory system. We study microRNAs (miRNAs), which serve as post-transcriptional regulators of gene expression. Our approaches are to develop novel methods for functional analysis, to identify target genes, and to unravel the physiological roles of miRNAs.
(A) The expression patterns of mir-1 miRNA detected by a whole-mount in situ hybridization method that we developed. (B) The expression patterns of a mir-1 promoter::GFP reporter transgene. The coincidence between the expression patterns indicates the absence of post-transcriptional regulation of the miRNA during biogenesis.
Andachi, Y., and Kohara, Y. (2018). MicroRNA detection by whole-mount in situ hybridization in C. elegans. Methods Mol Biol 1680, 75-86.
Andachi, Y., and Kohara, Y. (2016). A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans. RNA 22, 1099-1106.
Hamashima, K., Mori, M., Andachi, Y., Tomita, M., Kohara, Y., and Kanai, A. (2015). Analysis of genetic code ambiguity arising from nematode-specific misacylated tRNAs. PLoS One 10, e0116981.