Radiation is a subject of research to investigate effects on living organisms, and is used for labels in various fields of life science. The Radioisotope Unit is responsible for the administration of radiation, and provides support for studies that take advantage of radioisotopes.
In Caenorhabditis elegans sophisticated methods are available to determine gene expression patterns and to generate genetically modified strains, which are indispensable for reverse genetic analysis. Aiming at the elucidation of gene regulatory system, we study microRNAs, which serve as post-transcriptional regulators of gene expression. Our approaches are to develop novel methods for functional analysis, to identify target genes, and to unravel the physiological roles of microRNAs.
Survey meters and files used for the administration of radiation and radioisotopes.
Andachi Y, Kohara Y. MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans. Methods Mol Biol. 2018;1680:75-86.
Andachi Y, Kohara Y. A whole-mount in situ hybridization method for microRNA detection in Caenorhabditis elegans. RNA. 2016 Jul;22(7):1099-106.
Hamashima K, Mori M, Andachi Y, Tomita M, Kohara Y, Kanai A. Analysis of genetic code ambiguity arising from nematode-specific misacylated tRNAs. PLoS One. 2015 Jan 20;10(1):e0116981.
Andachi Y. A novel biochemical method to identify target genes of individual microRNAs: identification of a new Caenorhabditis elegans let-7 target. RNA. 2008 Nov;14(11):2440-51.