Maeshima Group • Genome Dynamics Laboratory::National Institute of Genetics

Maeshima Group • Genome Dynamics Laboratory

3D-organization and dynamics of human genome

Faculty

MAESHIMA, Kazuhiro

Professor

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Research Summary

Our research interest is to know how a long string of human genome is three-dimensionally organized in the cell, and how the human genome is read out for cellular proliferation, differentiation and development. For this purpose, we are using a unique combination of molecular cell biology and biophysics, such as live-cell single molecule imaging, superresolution imaging, genomics and computational simulation.

Human genome chromatin consists of irregularly folded 10-nm fibers and forms numerous chromatin domains in the cell nuclei. The chromatin domain dynamically behaves like “liquid”. NPC, nuclear pore complex; NE, nuclear envelope.

Selected Publications

Hibino K, Sakai Y, Tamura S, Takagi M, Minami K, Natsume T, Shimazoe MA, Kanemaki MT, Imamoto N, Maeshima K. Single-nucleosome imaging unveils that condensins and nucleosome-nucleosome interactions differentially constrain chromatin to organize mitotic chromosomes. Nat Commun. 2024 Aug 21;15(1):7152.

Iida S, Ide S, Tamura S, Sasai M, Tani T, Goto T, Shribak M, Maeshima K. Orientation-independent-DIC imaging reveals that a transient rise in depletion attraction contributes to mitotic chromosome condensation. Proc Natl Acad Sci U S A. 2024 Sep　3;121(36):e2403153121.

Maeshima K, Iida S, Shimazoe MA, Tamura S, Ide S. Is euchromatin really open in the cell? Trends Cell Biol. 2024 Jan;34(1):7-17.

Nozaki T, Shinkai S, Ide S, Higashi K, Tamura S, Shimazoe MA, Nakagawa M, Suzuki Y, Okada Y, Sasai M, Onami S, Kurokawa K, Iida S, Maeshima K. Condensed but liquid-like domain organization of active chromatin regions in living human cells. Sci Adv. 2023 Apr 5;9(14):eadf1488.