Experimental Technique
Experimental Technique (by Laboratories)
Genome Evolution • Kurokawa Group
- Technique for predicting environmental temperature based on metagenomic sequence（Metagenomic Thermometer)
- Fast and accurate taxonomic assignment tool of metagenomic data（VITCOMIC2）
- Latent environment allocation of microbial community data（LEA）
- Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
Genome Diversity • Mori Group
- Technique for predicting environmental temperature based on metagenomic sequences（Metagenomic Thermometer)
- Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
Genome Informatics • Nakamura Group
- Practical Assembly Guideline for Genomes with Heterozygosity
- ‘one-click’ identification of causative on web（Mudi）
- Prokaryotic genome annotation pipeline for data submission to DDBJ（DFAST）
Brain Function • Hirata Group
- Neuronal birthdate tagging method
- “NeuroGT Database”, a brain atlas of neurogenic tagging CreER drivers（NeuroGT）
Molecular and Developmental Biology • Kawakami Group
- Efficient production of recombinant proteins using the Tol2 transposon *Joint research with Kyowa Kirin Co., Ltd
- Seeing the visual world in the brain in real time
- Simple and efficient multiple sgRNA expression system in zebrafish
- Zebrafish optogenetics illuminates an ALS mystery (Review)* Collaboration with Tokyo Medical University
- Virtual reality system to analyze neural activity and behavior in adult zebrafish
Mouse Genomics Resource • Koide Group
- Genome editing in wild mouse strains
- Development of a freeware that enables automatic characterization of social behavior in mice（DuoMouse）
Symbiosis and Cell Evolution • Miyagishima Group
Microbial Physiology • Niki Group
- High efficient cloning technology using iVEC method
Plant Cytogenetics • Nonomura Group
- Visualization of autophagy
- 3D analysis of complex structures in living things
- (Research paper)(You can see the 3D images of several organisms.)
Mammalian Development • Saga Group
- Professor Saga retired in April 2022.
- Simple non-arbitrary method to quantify fluorescent images
- Genetics without making mouse lines
Model Fish Genetics • Sakai Group
- Establishment of a zebrafish inbred strain
- In vitro storage of functional sperm at room temperature in zebrafish and medaka
- Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.
Multicellular Organization Laboratory • Sawa Group
- Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
Mammalian Genetics • Shiroishi Group
- Now operated at RIKEN BRC
- User-friendly NIG mouse genome database（NIG_MoG
） - Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the public（NIG_MoG2）
DNA Data Analysis • Ikeo Group
- One-stop platform for NGS data analysis（Maser）
Human Genetics • Inoue Group
- Comprehensive discovery of CRISPR-targeted terminally redundant sequences
Molecular Cell Engineering • Kanemaki Group
- AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
- Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
- Simple method for generation of yeast conditional mutants by use of the AID technology
- New toolkit for tagging and a degradation inhibitor for the AID system
Genome Dynamics • Maeshima Group
- Development of a New Method for Synthesis of Tandem Hairpin Pyrrole (Cultured cell) (Tissue section)
- Method for telomere-specific chromatin capture
Database Center for Life Science （DBCLS)
- CRISPRdirect — Rational design of CRISPR/Cas target
- Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D
- These tools are developed jointly with the National Bioscience Database Center (NBDC) of JST.
Experimental Technique (by Categories)
-
Observation
-
Analysis
-
Modification of Gene
-
Development of bioresources
-
DNA recombination
-
Cell Culture
-
Analytical Tool
-
Database
Observation
- Seeing the visual world in the brain in real time
- Development of a New Method for Synthesis of Tandem Hairpin Pyrrole(Cultured cell) (Tissue section)
- 3D analysis of complex structures in living things
- (Research paper)(You can see the 3D images of several organisms.)
- Visualization of autophagy
- Zebrafish optogenetics illuminates an ALS mystery (Review) * Collaboration with Tokyo Medical University
- Virtual reality system to analyze neural activity and behavior in adult zebrafish
Analysis
- Practical Assembly Guideline for Genomes with Heterozygosity
- Method for telomere-specific chromatin capture
- Comprehensive discovery of CRISPR-targeted terminally redundant sequences
Modification of Genes
- Protein Knockdown Method in Photosynthetic Organisms
- AID2 (an improved AID system) enables rapid target degradation in yeast, mammalian cells, and mice (protocol)(review)
- Method for rapid protein depletion in nematode individuals using an improved auxin-inducible degron (AID2) system
- Simple method for generation of yeast conditional mutants by use of the AID technology
- Genome editing in wild mouse strains
- Genome editing in wild mouse strains
- High efficient cloning technology using iVEC method
- Genetics without making mouse lines
- New toolkit for tagging and a degradation inhibitor for the AID system
- Simple and efficient multiple sgRNA expression system in zebrafish
- Neuronal birthdate tagging method
Development of bioresources
DNA recombination
- Efficient production of recombinant proteins using the Tol2 transposon*Joint research with Kyowa Kirin Co., Ltd
Cell Culture
- In vitro storage of functional sperm at room temperature in zebrafish and medaka
- Differentiation of zebrafish spermatogonial stem cells to functional sperm in culture.
Analytical Tool
- Technique for predicting environmental temperature based on metagenomic sequences（Metagenomic Thermometer)
- Fast and accurate taxonomic assignment tool of metagenomic data（VITCOMIC2）
- Latent environment allocation of microbial community data（LEA）
- Ultra-fast amino acid sequence similarity search server against public metagenomes（PZLAST）
- ‘one-click’ identification of causative on web（Mudi）
- Development of a freeware that enables automatic characterization of social behavior in mice（DuoMouse）
- Simple non-arbitrary method to quantify fluorescent images
- One-stop platform for NGS data analysis（Maser）
- Prokaryotic genome annotation pipeline for data submission to DDBJ（DFAST）
- CRISPR/Cas9のためのガイドRNA設計ソフトウエア（CRISPRdirect）※
- CRISPRdirect — Rational design of CRISPR/Cas target
Database
- Metabolome databases for analyzing sample-specific unknown metabolites（FoodMR ）（PlantMR ）（ThingMR ）
- User-friendly NIG mouse genome database（NIG_MoG）
- Release of an upgraded NIG-Mouse Genome Database, “NIG_MoG2”, to the public（NIG_MoG2）
- “NeuroGT Database”, a brain atlas of neurogenic tagging CreER drivers（NeuroGT）
- Reference expression dataset -40 organs dataset, 4 different methods, with BodyParts 3D*
- Database Center for Life Science （DBCLS)