One of the main fields in molecular biology is DNA replication, which originated from the proposal of the DNA double-helix structure by James Watson and Francis Crick in 1953, followed by the isolation of DNA polymerase I by Arthur Kornberg in 1958.
Notably, Japanese scientists have played important roles in this field since the early days. The most famous example is Reiji Okazaki of Nagoya University, who identified Okazaki fragments on lagging strands. Yukinori Hirota and Seiichi Yasuda at National Institute of Genetics (NIG) played a significant role in identifying the replication origin, oriC, in the E. coli chromosome. The 6th NIG director, Junichi Tomizawa, revealed the replication mechanism of the colE1 plasmid. More recently, Hiroyuki Araki at NIG identified multiple proteins essential for initiating DNA replication in budding yeast and showed the initiation mechanism controlled by S-CDK.
The field is now heading to the in vitro reconstitution using purified eukaryotic proteins, which will eventually reveal mechanistic details of how DNA replication takes place. From the biomedical point of view, a failure of chromosome replication leads to chromosome instability, which is highly related to cancer formation and genetic disorders. However, DNA replication has been mainly studied with unicellular organisms such as budding yeast. Therefore, the mechanism in mammalian cells remains elusive. Moreover, the relation to transcription, chromatin environment, 3D chromosome organization, and development is poorly understood. In the NIG international symposium, we wish to discuss the latest results in DNA replication and related issues.
- Schedule
- Nov. 11 and 12, 2022
- Venue
- NIG Lecture Hall
- Registration
- Please applicate at the registration form.
*Deadline of registration is Friday, October 14. - Participation Fee
- Free to attend
＊ A party is planned on November 11
| Fumio Hanaoka | National Institute of Genetics, Japan |
| Hiroyuki Araki | National Institute of Genetics, Japan |
| John Diffley | The Crick Institute, UK |
| Thomas Kunkel | National Institute of Health, USA |
| David Gilbert | San Diego Biomedical Research Institute, USA |
| Chun-Long Chen | Institut Curie, France |
| Cheng-Fu Kao | Academia Sinica, Taiwan |
| Fumiko Esashi | University of Oxford, UK |
| Hisao Masai | Tokyo Metropolitan Institute of Medical Science, Japan |
| Tsutomu Katayama | Kyushu University, Japan |
| Seiji Tanaka | Kochi University of Technology, Japan |
| Takehiko Kobayashi | University of Tokyo, Japan |
| Tomomi Tsubouchi | National Institute for Basic Biology, Japan |
| Yasukazu Daigaku | Cancer Institute, Japan |
| Tatsuro Takahashi, | Kyushyu University, Japan) |
| Kazuhiro Maeshima | National Institute of Genetics, Japan |
| Yasuto Murayama | National Institute of Genetics, Japan |
| Masato Kanemaki | National Institute of Genetics, Japan |
Nov.11,2022
| 10:00 | Opening remarks |
| 10:10 | Introductionby Director Hanaoka (NIG) (30 min)
|
| 10:40 | Talk❶Kunkel (NIH) (50 min) Determinants of eukaryotic nuclear DNA replication fidelity |
| 11:30 | Talk❷Takahashi (Kyushu University) (30 min) The mechanism of a chromatin-remodeling reaction associated with replication error correction |
| 12:00 | Lunch |
| 13:00 | Talk❸Maeshima (NIG) (30 min) Liquid-like domain organization of early DNA replication regions in living human cells |
| 13:30 | Talk❹Kao (Academia Sinica) (30 min) Negotiating DNA replication/repair with chromatin |
| 14:00 | Talk❺Diffley (Crick Institute) (50 min) The role of the DNA damage checkpoint at stalled replication forks |
| 14:50 | Poster with coffee (50 min)
|
| 15:40 | Talk❻Araki (NIG) (50 min) Assembly of replication machinery at origins in budding yeast |
| 16:30 | Talk❼Murayama (NIG) (30 min) How cohesin responds to DNA replication |
| 17:00 | Talk❽Katayama (Kyushu University) (30 min) Dynamic nucleoprotein complexes sustaining regulation for the chromosomal replication initiation in escherichia coli |
| 17:30 | Talk❾Masai (TMIMS) (30 min) Transcription can drive replication |
| 19:00 | Dinner at Fujisan Tokyu Hotel |
Nov.12,2022
| 9:00 | Talk❿Tanaka (Kochi University of Technology) (30 min) Identification of atypical replication origin in the metallothionein-encoding repeats in the budding yeast Saccharomyces cerevisiae |
| 9:30 | Talk⓫Kobayashi (The University of Tokyo) (30 min) rDNA stability and lifespan in yeast |
| 10:00 | Talk⓬Daigaku (Cancer Institute of JFCR) (30 min) Genome-wide profiling of DNA polymerase usage and replication kinetics in human cells |
| 10:30 | Break (10 min) |
| 10:40 | Talk⓭Kanemaki (NIG) (30 min) Temporal distribution of the replisomes and initiation zones in human cells |
| 11:10 | Talk⓮Gilbert (San Diego BioMed) (50 min) Early replication control elements are developmentally-regulated enhancers of replication initiation |
| 12:00 | Lunch |
| 13:00 | Talk⓯Chen (Institut Curie) (30 min) Decipher DNA replication program of human cells at the single-molecule and single-cell levels |
| 13:30 | Talk⓰Tsubouchi (NIBB) (30 min) Understanding plasticity of mammalian DNA replication through studies of mouse ES cells |
| 14:00 | Talk⓱Esashi (University of Oxford) (30 min) Homologous recombination at human centromeres: Friend or foe? |
| 14:30 | Poster with coffee (50 min)
|
| 15:20 | Closing remark |
Registration is required for participation.
*Deadline of registration is Friday, October 14.
Registration Closed.
