Animals generate a range of behaviors depending on visual information that they receive from their outside world. Using zebrafish as a model, our lab studies the neural circuit mechanisms by which visual inputs produce goal-directed behavioral outputs. In particular, we aim to understand the roles of genetically defined neuron types and their circuit connectivity underlying the visually guided behaviors. The approaches that our lab uses include behavioral, genetic and optical techniques, as well as quantitative data analyses.
(A) A larval zebrafish at 5 days post fertilization. Arrows indicate visual motion stimuli presented to the zebrafish. (B) Calcium imaging and quantitative analysis reveal the activity pattern of multiple neuron types in a brain region (e.g. pretectum) involved in the visual processing. (C) Predicted wiring diagram of the whole-field motion processing circuit in the pretectum.
Kramer A, Wu Y, Baier H, Kubo F. Neuronal Architecture of a Visual Center that Processes Optic Flow. Neuron. 2019 Jul 3;103(1):118-132.e7.
Förster D, Kramer A, Baier H, Kubo F. Optogenetic precision toolkit to reveal form, function and connectivity of single neurons. Methods. 2018 Nov 1;150:42-48.
Förster D, Arnold-Ammer I, Laurell E, Barker AJ, Fernandes AM, Finger-Baier K, Filosa A, Helmbrecht TO, Kölsch Y, Kühn E, Robles E, Slanchev K, Thiele TR, Baier H, Kubo F. Genetic targeting and anatomical registration of neuronal populations in the zebrafish brain with a new set of BAC transgenic tools. Sci Rep. 2017 Jul 12;7(1):5230.
Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron. 2016 Feb 17;89(4):725-33.