The centrosome is a conserved organelle that serves as the microtubule organizing center and regulates many biological processes. However, the mechanisms governing centrosome duplication and the underlying structural principles remained poorly understood and represent a long-lasting important question in biology.
question in biology. We mainly focus on understanding the mechanisms of centrosome biogenesis and dynamics by using the combination of innovative and multi-disciplinary approaches. We are investigating the following specific aims by using human cells, mouse and C. elegans as model systems.
(A) Cell cycle-dependent centriole formation. (B) SAS-6 homodimers dictate the universal 9-fold symmetry of centrioles. (C) HsSAS-6, human SAS-6, is required for centriole formation in human cells. Centrin: centriole marker.
Tsuchiya, Y., Yoshiba, S., Gupta, A., Watanabe, K., and Kitagawa, D. (2016). Cep295 is a conserved scaffold protein required for generation of a bona fide mother centriole. Nat Commun 7, 12567.
Shiratsuchi, G., Takaoka, K., Ashikawa, T., Hamada, H., and Kitagawa, D. (2015). RBM14 prevents assembly of centriolar protein complexes and maintains mitotic spindle integrity. EMBO J 34, 97-114.
Ohta, M., Ashikawa, T., Nozaki, Y., Kozuka-Hata, H., Goto, H., Inagaki, M., Oyama, M., and Kitagawa, D. (2014). Direct interaction of Plk4 with STIL ensures formation of a single procentriole per parental centriole. Nat Commun 5, 5267.