Regulation of the nuclear structure formation Nature Structural & Molecular Biology Volume: 17 　 Maeshima Laboratory, Biological Macromolecules Laboratory Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase Kazuhiro Maeshima, Haruki Iino, Saera Hihara, Tomoko Funakoshi, Ai Watanabe, Masaomi Nishimura, Reiko Nakatomi, Kazuhide Yahata, Fumio Imamoto, Tsutomu Hashikawa, Hideo Yokota & Naoko Imamoto Nature Structural & Molecular Biology, Volume: 17, Pages: 1065-1071 (2010). Published online: 15 August 2010 　 doi:10. 1038 /nsmb.1878 　Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus almost double during interphase in dividing cells. How are these events coordinated with the cell cycle? Recently, we reported, based on newly developed techniques for visualizing NPC formation, that cyclin-dependent kinases (Cdks) promote interphase NPC formation in human dividing cells. Cdks seem to drive an early step of NPC formation because Cdk inhibition suppressed generation of 'nascent pores', which we argue are immature NPCs under the formation process. Strikingly, Cdk suppression did not notably affect nuclear growth, suggesting that interphase NPC formation and nuclear growth have distinct regulation mechanisms. This work was performed as collaboration with Dr Imamoto, Dr Hashikawa, and Dr Yokota at RIKEN. The paper was published in Nature Structural & Molecular Biology and highlighted on the cover of September issue. 　 Nuclear volume and the number of nuclear pore complexes (NPCs) on the nucleus　 almost double during interphase in dividing cells (Right) . Nuclear pore formation but not nuclear growth is governed by cyclin-dependent kinases (Cdks) during interphase during cell cycle. The left SEM image is 'nascent pores', which we argue are immature NPCs under the formation process. ←Back