Mib-Jag1-Notch signaling regulates patterning and structural roles of the notochord by controlling cell-fate decisions
Development
Kawakami Laboratory, Division of Molecular and Developmental Biology
Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions
Mai Yamamoto, Ryoko Morita, Takamasa Mizoguchi, Hiromi Matsuo, Miho Isoda, Tohru Ishitani, Ajay B. Chitnis, Kunihiro Matsumoto, J. Gage Crump, Katsuto Hozumi, Shigenobu Yonemura, Koichi Kawakami and Motoyuki Itoh.
Development, 137, 2527-2537. (2010)@doi:10.1242/dev.051011


@During midline development in vertebrates, the notochord plays roles both in the patterning of its surrounding tissues and in the formation of the axial structure. However, how these roles are coordinated has been unknown. Here, we discover the Notch signaling regulates the patterning activity and the structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and the Mib-mediated ubiquitylation of Jagged is essential to activate Notch signaling. In zebrafish, loss and gain-of-function analyses showed that Mib-Jag1-Notch signaling accelerates the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. The abnormal non-vacuolated cell development leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signaling-mediated cell-fate determination.
(This work is carried out in collaboration with Itoh lad at Nagoya University)


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@ Non-vacuolated (red, arrowhead) and vacuolated (green, arrow) cells in the zebrafish notochord.


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