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CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells.
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| EMBO J., 15 August 2001 |
| Fukagawa group, Laboratory of Evolutionary Genetics |
CENP-H, a constitutive centromere component, is required for centromere targeting of CENP-C in vertebrate cells.
Fukagawa T, Mikami Y, Nishihashi A, Regnier V, Haraguchi T, Hiraoka Y, Sugata N, Todokoro K, Brown W, Ikemura T.
EMBO J., 20, 4603-4617 (2001)
CENP-H has recently been discovered as a constitutive component of the centromere that co-localizes with CENP-A and CENP-C throughout the cell cycle. The precise function, however, remains poorly understood. We examined the role of CENP-H in centromere function and assembly by generating a conditional loss-of-function mutant in the chicken DT40 cell line. In the absence of CENP-H, cell cycle arrest at metaphase, consistent with loss of centromere function, was observed. Immunocytochemical analysis of the CENP-H-deficient cells demonstrated that CENP-H is necessary for CENP-C, but not CENP-A, localization to the centromere. These findings indicate that centromere assembly in vertebrate cells proceeds in a hierarchical manner in which localization of the centromere-specific histone CENP-A is an early event that occurs independently of CENP-C and CENP-H.
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Immunofluorescence analysis of CENP-H-deficient cells. Interphase cells were stained with anti-CENP-H, anti-CENP-A and anti-CENP-C antibodies. Antibody signals were detected with Cy3-conjugated secondary antibodies (red). DNA was counterstained with DAPI (blue). The scale bars correspond to 10 μm.
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Entrez PubMed |
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