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The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish
Development 137, 3257-3268.
Kawakami Laboratory, Division of Molecular and Developmental Biology

The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish.
Imai, F., Yoshizawa, A., Fujimori-Tonou, N., Kawakami, K., and Masai, I.
Development, 137, 3257-3268. (2010)  DOI: 10.1242/dev.053124

   In the vertebrate lens development, epithelial cells differentiate into fiber cells, which are elongated and flat in shape and form a multilayered lens fiber core. The zebrafish volvox ( vov ) mutant shows defects in lens fiber differentiation. In the vov mutant, lens epithelial cells fail to proliferate properly and differentiating lens fiber cells do not fully elongate. We found that the vov mutant gene encodes a subunit of the 26S proteasome. The proteasome regulates degradation of polyubiquitylated proteins. Thus, the ubiquitin proteasome system is required for cell proliferation of lens epithelium and for the differentiation of lens fiber cells in zebrafish.

(This work is carried out in collaboration with Masai lab at Okinawa Institute of Science and Technology)

Figure1
Visualization of lens fiber cells by using transgenic zebrafish.
(Left) Wild type zebrafish. (Right) vov mutant zebrafish.
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